Abstract
Chicken infectious anemia (CIA) is a poultry disease that causes huge economic losses in the poultry industry worldwide. Commercially available CIA vaccines are derived from wild-type chicken anemia viruses (CAVs) by serial passage in cells or chicken embryos. However, these vaccinal viruses are not completely attenuated; therefore, they can be transmitted vertically and horizontally, and may induce clinical symptoms in young birds. In this study, we sought to eliminate these issues by developing a subunit vaccine exploiting the CAV structural proteins, engineering recombinant baculovirus-infected Spodoptera frugiperda (Sf9) cells that contained both the viral protein 1 (VP1) and VP2 of CAV. Moreover, we produced single-chain chicken interleukin-12 (chIL-12) in the same system, to serve as an adjuvant. The recombinant VP1 was recognized by chicken anti-CAV polyclonal antibodies in Western blotting and immunofluorescence assays, and the bioactivity of the recombinant chIL-12 was confirmed by stimulating interferon-γ (IFN-γ) secretion in chicken splenocytes. Furthermore, the ability of the recombinant VP1 to generate self-assembling virus-like particles (VLPs) was confirmed by transmission electron microscopy. Specific pathogen-free (SPF) chickens inoculated with VLPs and co-administered the recombinant chIL-12 induced high CAV-specific antibodies and cell-mediated immunity. Taken together, the VLPs produced by the baculovirus expression system have the potential to be a safe and effective CIA vaccine. Finally, we demonstrated the utility of recombinant chIL-12 as an adjuvant for poultry vaccine development.
Highlights
Chicken infectious anemia (CIA) causes severe economic losses in the poultry industry worldwide.The causative pathogen, chicken anemia virus (CAV), induces clinical anemia in young chicks when they lack protective maternal antibodies [1]
An approximately 50-KDa protein was detected in the cell lysates of B-viral protein 1 (VP1)-/B-VP2-infected Sf9 antibodies as approximately probes
AsAs a result, a safe, high quality, and economical vaccine is needed in order to improve chicken a result, a safe, high quality, and economical CIA vaccine is needed in order to improve chicken production
Summary
The causative pathogen, chicken anemia virus (CAV), induces clinical anemia in young chicks when they lack protective maternal antibodies [1]. CAV, a non-enveloped DNA virus, is a member of the Gyrovirus genus and the family of Anelloviridae [4]. The genome of CAV is a circular, single-stranded, negative-sense DNA approximately. VP1, the major structural capsid protein, plays an important role in antigenicity, and induces neutralization antibodies in hosts [8]. The co-expression of VP1 and VP2 in the same cells is necessary for recombinant VP1 to form correctly and induce neutralizing antibodies in inoculated chickens [9,11]. VP3, known as apoptin, induces apoptosis in chicken thymocytes and lymphoblastoid T cells [12]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
