Abstract
Membrane protein reconstituted cationic liposomes are constructed using cell-free membrane protein synthesis in the presence of cationic liposomes. The chaperon effect of cationic liposomal membrane assists in folding the functional conformation of membrane protein. This preparation method enables the provision of the usage of proteoliposomes for drug delivery.
Highlights
Membrane protein reconstituted cationic liposomes are constructed using cell-free membrane protein synthesis in the presence of cationic liposomes
Membrane proteins have been increasingly studied for their use in advanced applications in the drug delivery system (DDS) eld, including for membrane protein-conducted drug delivery[1] and for delivery of the membrane protein itself into the plasma membrane.[2]
We report the preparation of cationic proteoliposomes using dioleoyl-sn-glycero-3ethylphosphocholine chloride salt (DOEPC) and a cell-free membrane protein synthesis/liposome system and describe the chaperoning effect of these cationic liposomes, in which Cx43 was selected as a model membrane protein
Summary
Membrane protein reconstituted cationic liposomes are constructed using cell-free membrane protein synthesis in the presence of cationic liposomes. To evaluate the effect of the positive charge of the liposomes on cell-free membrane protein synthesis, we prepared cationic When DOEPC was 4 mol%, the cationic liposomes inhibited the cell-free Cx43 synthesis as shown by the yield of (70.1 Æ 17.6) %.
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