Abstract
Melanin nanoparticles (MNP) were isolated from sepia ink, spherical and range in size from 40 to 160 nm. Carrageenan-based nanocomposite films with different concentration of MNP (0, 0.25, 0.5, 1.0, and 2.0 wt%) were prepared and their physical, thermal and UV-barrier properties were evaluated. Field emission scanning electron (FE-SEM) micrographs showed well-dispersed MNPs in the carrageenan matrix. The addition of MNP completely shielded the UV light and also affected the apparent color and transparency of the nanocomposite film. The addition of up to 1.0% by weight of MNP significantly increased the mechanical strength of the carrageenan film. The heat resistance of the carrageenan film also increased by mixing with MNP. Incorporation of MNP also reduce the water barrier property and increase the hydrophobicity of the carrageenan film. Also, the MNP-incorporated carrageenan nanocomposite films showed strong antioxidant activity with some antimicrobial activity against foodborne pathogenic bacteria, L. monocytogenes and E. coli.
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