Preparation of Capsaicin Molecular Imprinting Polymers and Application to Solid Phase Extraction

Abstract

Molecular imprinting polymers(MIPs) were synthesized by bulk polymerization,using capsaicin as the template molecule and 2-vinylpyridine(2-VP) and Acrylamide(AM) as the function monomers.The specific adsorption ability of the molecularly imprinted polymers(MIPs) to capsaicin was evaluated by equilibrium binding experiments,saturated adsorption time was assessed by saturated adsorption experiments and selective adsorption ability to capsaicin analogs was appraised by selective adsorption experiments.The results showed that MIPs exerted the best adsorption efficiency to capsaicin when the ratio of capsaicin:2-VP:AM was 1:2:2,with a adsorption capacity of 20.46 mg/g and a recognition factor of 3.05.Morevoer saturated adsorption time was 6 h and better selective recognition to capsaicin was found.In this study,the elution efficiencies of eluents of different polarity for specific adsorption and non-specific adsorption of capsaicin in solid phace extraction column of MIPs were investigated.The specific adsorption was kept while the non-specific adsorption was discharged to the greatest extent when using chloroform as the eluent.