Preparation of capillary columns coated with linear polymer containing hydrophobic and charged groups for capillary electrochromatography.

Abstract

A linear polymer-coated capillary was prepared by in-capillary copolymerization of N-tert-butylacrylamide (TBAAm) with a charged monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), after the capillary pretreatment with a bifunctional reagent. The coated capillaries were applied in capillary electrochromatographic (CEC) separation of small neutral compounds. Hydrophobic groups in the linear polymer, which were immobilized onto the capillary surface, functioned as the stationary phase in reversed-phase CEC separation, and charged groups in the linear polymer generated electroosmotic flow (EOF) along the column. The coated capillaries were prepared by a simple procedure. Moreover, the reproducibility with respect to EOF rate and migration times of the solutes was excellent. The results for CEC separation of small molecules using the linear polymer-coated capillaries are presented.