Preparation of biokit to treatment the Petrol spots in water

Abstract

Microorganism isolates used in plant protection were obtained from the College ofAgriculture / University of Baghdad. Its purity was assured. The microbial biokit was preparedthrough using several unsatisfactory and environmentally friendly isolate. The bioactivity ofbiokit were studied in laboratory and showed susceptibility in removing contaminants. Theresults showed that the biomass of the isolates prior to the loading process on a mediumcontaining crude oil as a single carbon source of 5 g/ L, which indicates growth ability andefficiency in the decomposition of pollutants when increase the isolates biomass more than 3 g /L, as well as the appearance of turbidity in the media compared to the control sample (mediawithout oil), and obtained bioemulsifier with a concentration of 2.3 g / L. The chemical analysisof the samples under study was carried out using Gas Chromatography (GC) and GC-MScompared with a non-treatment (control).
 The results showed the appearance of 43 topographic peaks each indicating a chemicalcompound in the control sample, while a number of these peaks disappeared and a space wasreduced in other peaks that refers to the concentration of compounds, in the biomass sample, andreached the number of peaks in 26, indicating the efficiency of the biokit in the oildecomposition and dismantling of hydrocarbons. The increase in the number of livemicroorganisms and the incubation period is more than 28 days by increasing the growthintensity in the third week may be due to decomposing oil compounds and their exploitation innutrition, where crude oil provides a source of carbon and energy for microorganism’s isolates.In other results showed that the mass of crude oil was converted to a mass of gel andemulsification compared to the control sample after seven days period at a temperature of 28±2°C, Which is characterized by low toxicity and easy decomposition and reduces the surfacetension of hydrocarbons and makes them more solubility and preparation for the dismantling oflife and increase the rate of disintegration. The biokit was tested for retention in storageconditions for a period of one year.
 at alone prior to the production of the biokit, which showed poor storage capacity. We concludethat biomass has the potential to treat hydrocarbons (oil contaminants) in the environment.