Abstract
ZnO electrospun nanofibers can act as seed fibers to fabricate multidentate barbed fibers perpendicular to the growth of the fibers using the chemical bath deposition (CBD) method. Fibers with a multirod morphology have a porous grid structure. The sample is easy to recover, and the nonpolar surface in the sample is sufficiently exposed. In the research of barbed fiber fabrication and adsorption on bovine serum albumin (BSA), the effects of different chemical bath conditions on the growth of ZnO nanorods were discussed. Barbed fibers with large slenderness ratios were obtained at a water content of 60 mL at 75 °C. Each milligram of barbed fibers can quickly adsorb about 162 μg of protein within 30 min. The adsorption activity of BSA between polar and nonpolar ZnO surfaces was also studied. The selective adsorption behavior of BSA on the nonpolar surface was revealed.
Highlights
Serum albumin in bovine serum (BSA) concentrate is a protein that is commonly used in immunodiagnostic procedures, clinical reagents, cell culture media, and protein chemistry in biological laboratories.[1]
The results showed that ZnO had specific adsorption for new and formed proteins
XRD patterns indicate that both seed fibers and barbed fibers are composed of the wurtzite ZnO structure
Summary
Serum albumin in bovine serum (BSA) concentrate is a protein that is commonly used in immunodiagnostic procedures, clinical reagents, cell culture media, and protein chemistry in biological laboratories.[1] The adsorption of proteins on the surface of biological materials is an important step in the basic biological process. The results showed that ZnO had specific adsorption for new and formed proteins. To characterize the adsorption mechanism between the protein and the material surface, centrifugal treatment was needed to separate the powders from the protein solution. ZnO grown on substrates in the study of protein adsorption has been widely reported.[11,12] Limited by the morphology of the sample, the two-dimensional ZnO material grown on the substrate cannot have a high specific surface area. Wang et al found that compared with other morphologies, ZnO nanorods have the best adsorption performance for BSA
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