Abstract
The vesicles (proteoliposomes) were artificially prepared by sonic oscillation from hydrophobic protein, which was isolated from cytoplasmic membrane fraction of Bacillus subtilis W-23, and phospholipids or lipids, which were isolated from different organims; phospholipids from hen-egg yolk and soybean, and lipids from B . subtilis W-23. In the vesicles, remarkable activity of L-alanine uptake was demonstrated in the presence of NADH or ascorbate-phenazine methosulfate as an energy source. The uptake activity was temperature-dependent and inhibited by respiration inhibitor (KCN) and uncoupler (ClCCP). Replacement experiment was also performed.
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