Preparation of Antioxidant Hydrolysates from Round Scad (<i>Decapterus maruadsi</i>) Protein by Composite Enzymatic Hydrolysis

Abstract

Round scad (Decapterus maruadsi) was hydrolyzed by composite enzymatic hydrolysis to obtain the hydrolysates possessing high antioxidant activity. The optimum conditions of antioxidant hydrolysates preparation by composite enzymatic hydrolysis were investigated. The results demonstrated that superior antioxidant activities (low IC50 values of radical scavenging activity and high reducing power) were obtained at an enzyme (a mixture of neutral protease and trypsin, 1:1 w/w) to substrate ratio of 0.3%, an incubation time of 5 h and an incubation temperature of 50 °C. Under the conditions, the recovery ratio of protein was 75.24% and the degree of hydrolysis was 25.86%