Preparation of Antibiotics-containing Electrospinning Chitosan/Gelatin Nanofibers

Abstract

The electrospun nanofibers-based systems have opened new windows on drug delivery systems. Antibiotic treatment and drug resistance in infectious disease management have introduced some potential applications of using electrospun nanofibers. In this respect, chitosan/gelatin nanofibers can be considered a reliable source for drug delivery. This study aimed to synthesize antibiotics-containing electrospinning chitosan/gelatin nanofibers. To this end, electrospinning was performed using different concentrations of chitosan and gelatin, followed by adding ceftriaxone to the corresponding solutions. Afterward, nanofibers were crosslinked through ceftriaxone loading. The nanofiber was characterized in terms of its morphology via scanning electron microscopy (SEM) analyses. In addition, spectrophotometrical analysis was performed to examine the amount of released ceftriaxone at 280 nm wavelength. Eventually, SPSS version 21 was used to perform statistical analyses. Based on the obtained results, the mean Gelatin diameters before and after crosslinking were 154.5±286 and 162.3±27, respectively; the mean Chitosan/gelatin (95:5) diameters before and after crosslinking were 192±25 and 208±11.6 respectively. Finally, the mean Chitosan/gelatin (80:20) diameters before and after crosslinking were 188.2±34.7 and 212.1±54.8, respectively. Overall, the obtained results indicate that the fiber diameters increase after crosslinking and by adding chitosan. This increase can be attributed to polymer chain entanglement in the gelatin chain reaction with glutaraldehyde’s aldehyde group. In addition, despite the short drug release time (i.e., 240 min). In general, chitosan lowered the drug release rate in comparison to gelatin nanofibrous. In conclusion, the fiber diameters rise with adding chitosan, followed by its glutaraldehyde crosslinking. The nanofibers showed a considerably higher drug release compared to polymeric films. Nevertheless, gelatin nanofibers such as chitosan decline the ceftriaxone release rate compared to gelatin nanofibers. Furthermore, prolonging the buffer solution immersion time enhances the drug release percentage.