Preparation of an Environmentally Friendly Nano-Insecticide through Encapsulation in Polymeric Liposomes and Its Insecticidal Activities against the Fall Armyworm, Spodoptera frugiperda

Abstract

Simple SummaryPests are an important factor that causes a heavy loss in corn yield and quality. The fall armyworm (FAW), Spodoptera frugiperda, is a newly invasive and extremely destructive pest, and it poses a major threat to agricultural production in China. While chemical pesticides are considered effective means for controlling the outbreak of destructive pests, pesticide delivery systems, such as microcapsules or nanoparticles, are an effective way to promote the utilization rate of traditional pesticides and to reduce environmental pollution. Therefore, the aim of this study is to design an environmentally friendly nano-insecticide that can enhance foliar retention and increase insecticidal activity. For this purpose, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG2000-NH2) was chosen to formulate the insecticide nanoparticles. The physicochemical properties were characterized and investigated in indoor and field efficacy trials. The results demonstrate that the nanoparticles hold promise for pest control. The insecticide emamectin benzoate (EB) was formulated with nanoparticles composed of DSPE-PEG2000-NH2 by the co-solvent method to determine its adverse impacts on the environment and to reinforce its dispersion, adhesion, and biocompatibility. A good encapsulation efficiency (70.5 ± 1.5%) of EB loaded in DSPE-PEG2000-NH2 polymeric liposomes was confirmed. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and contact angle meter measurements revealed that the DSPE-EB nanoparticles had a regular distribution, spherical shape, and good leaf wettability. The contact angle on corn leaves was 47.26°, and the maximum retention was higher than that of the reference product. DSPE-EB nanoparticles had strong adhesion on maize foliage and a good, sustained release property. The efficacy trial showed that the DSPE-EB nanoparticles had a strong control effect on S. frugiperda larvae, with the LC50 of 0.046 mg/L against the third-instar S. furgiperda larve after 48 h treatment. All these results indicate that DSPE-EB nanoparticles can serve as an insecticide carrier with lower environmental impact, sustained release property, and effective control of pests.