Abstract
Control of glucose concentration has tremendous significance in medical diagnosis, pharmaceuticals, food, and fermentation industries. Herein, we report on the fabrication of a facile, low‐cost, and sensitive enzyme‐based amperometric sensor using the electrochemically deposited polyaniline (PANI) film on a graphite electrode. PANI was deposited from an aqueous solution of 0.2 M aniline in 1.0 M hydrocholoric acid (HCl) by cyclic voltammetry (CV). Surface morphology and composition characterization of the PANI film were carried out by scanning electron microscopy (SEM), X‐ray photoelectron spectroscopy (XPS), and Fourier‐transform infrared (FTIR) spectroscopy. Potentiostatic immobilization of glucose oxidase (GOX) enzyme in the PANI film was carried out at 0.75 V to fabricate an amperometric glucose biosensor (GOx/PANI/graphite biosensor). The glucose concentration response of the prepared sensor was studied amperometrically by detecting hydrogen peroxide (H2O2). The detection of H2O2 was optimized by calibrating the effects of pH, reduction potential, and background current. A reduction potential of ‐0.4 V at pH 6 was the best combination to get a maximum amperometric response of the GOx/PANI/graphite biosensor. A stable current response was obtained in 4 min with a high reproducibility in linearity within the concentration range of 0.01 M‐0.1 M D‐glucose. Therefore, the fabricated GOx/PANI/graphite biosensor could be a promising candidate for glucose sensing.
Highlights
Glucose concentration measurement is immensely important in food processing, fermentation reactors, pharmaceuticals processes, textile industry, environmental monitoring, and clinical laboratories [1,2,3]
This paper describes a simple glucose biosensor fabricated by electropolymerization of aniline on graphite substrate and potentiostatic immobilization of glucose oxidase enzyme (GOx) onto the PANI film on the graphite surface
Analytical reagent grade aniline (C6H5NH2), glucose oxidase (GOx), sodium monohydrogen orthophosphate (NaH2PO4.2H2O), and D (+) glucose was purchased from Merck, USA
Summary
Glucose concentration measurement is immensely important in food processing, fermentation reactors, pharmaceuticals processes, textile industry, environmental monitoring, and clinical laboratories [1,2,3]. The response of a glucose biosensor requires enzyme immobilization on the electrode surface. The surface used for immobilization should facilitate a smooth transfer of an electron from a biocomponent to the electrode surface, and it should impart biocompatibility to the electrode surface [8]. It should enhance the response properties and be sensitive to perturbation so that a response is correctly recorded [8, 9].
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