Abstract
AbstractIn this article, we present a novel amino‐reserved magnetic chitosan microsphere (ARMCM) prepared from chitosan and Fe3O4 through inverse suspension crosslinking by formaldehyde and epichlorohydrin. The density of ARMCM was 1.47 g/mL, and the amino content was 2.44 mmol/g. The measurements data indicated that the saturation magnetization (σs) of ARMCM was 23.2 emu/g. The adsorption results showed that these magnetic chitosan microspheres had good adsorption capacity on endotoxin: the maximum absorption capacity was about 1792.1EU/g in 40 min; the endotoxin adsorption efficiency in the protein solution was close to 80% and what is more, protein adsorption efficiency was only 23.2 and 2.0%, respectively, for acidic and basic proteins. It is expected that this ARMCM will have a feasible and useful application in adsorbing endotoxin from biological products. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
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