Abstract
A facile one-pot synthesis of size-tunable mesoporous carboxyl-functionalized magnetic colloidal nanocrystal clusters (MCNCs) with high magnetization (82.0emu/g), large surface area (95m2/g), and excellent colloidal stability has been developed. The mesostructured MCNCs were synthesized by a solvothermal approach with iron (III) chloride hexahydrate as a precursor, ethylene glycol as a reducing agent, ammonium acetate as a porogen, and ethylenediaminetetraacetic acid disodium salt (EDTA-2Na) as a surface-modification agent. Glucoamylase was immobilized onto the mesoporous MCNCs via the different routes. These immobilized glucoamylase exhibited excellent thermal stability and reusability in comparison with the free enzyme. The residual activity of immobilized enzyme remained above 65% after 6h, while free glucoamylase was only left over 45% of the initial activity. And the residual activity of the immobilized enzyme was about 60% of the initial activity after the 10th reuse.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
