Abstract
The optimal conditions for the activation of Toyopearl by epichlorohydrin and subsequent immobilization of ligands were investigated. The optimal conditions for the activation were very different from those for agarose gel. The concentration of epoxy groups introduced was as high as 330 μmole per gram of wet gel for Toyopearl HW-55 and 150 μmole per gram of wet gel for Toyopearl HW-65 (diol type). Epoxy-activated Toyopearl was converted into amino and carboxyl derivatives and was subsequently coupled with various ligands. Glycamyl Toyopearl was prepared in a much shorter time (6 h) than agarose gel (800 h), because a higher reaction temperature could be used. The adsorbents obtained were successfully used for the affinity chromatography of lectin and trypsin. However, their adsorption capacities were lower than those of the agarose adsorbents prepared by the same methods.
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