Abstract
14C-labelled “active glycolic aldehyde” prepared by incubating 3- 14C-hydroxypyruvate with thiamine pyrophosphate and a preparation of pyruvate oxidase from pig heart muscle was isolated by ion exchange chromatography. The substance contains thiamine and 14C in the ratio of 1 : 1. It yields glycolic acid by enzymatic oxidation with 2,6-dichlorphenolindophenol. By analogy with “active acetaldehyde” it is proposed to be 2-(1,2-dihydroxyethyl)-thiamine pyrophosphate.
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