Abstract
This study deals with the preparation of surface-modified mesoporous activated carbon (SMMAC) using ethylenediamine and glutaraldehyde. Surface modification renders the covalent immobilization of acidic lipase (AL) onto MAC. The AL was produced from Pseudomonas gessardii using goat tallow as the substrate and purified. The maximum immobilization capacity of SMMAC for AL was 5440 U/g. The SMMAC and ALSMMAC were characterized for physico-chemical, morphological and material structure. The ALSMMAC showed credible hydrolytic efficiency toward slaughterhouse lipid wastes and vegetable oils. The hydrolysis of slaughterhouse lipid wastes and vegetable oils obeyed pseudo first order kinetic model. The excellent operational stability of ALSMMAC was verified using olive oil.
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