Abstract
The preparation of an antibody against acetochlor and its application on immunoaffinity chromatography (IAC) cleanup was investigated. The hapten was synthesized by reacting acetochlor with 3-mercaptopropionic acid and then linked with a carrier protein by the carbodiimide method. After immunizing a rabbit with hapten-bovine serum albumin (BSA) conjugate, the obtained antiserum showed an IC(50) value of 0.2 ng/mL. By coupling the purified polyclonal antibody with CNBr-activated Sepharose 4B, an IAC column was prepared. The dynamic column capacity was 5560 ng/mL gel. The IAC was then used to clean up a peanut sample solution, followed by high-performance liquid chromatography (HPLC) separation and ultraviolet (UV) detection. The recoveries of acetochlor from spiked peanut samples at levels of 5-100 microg/kg ranged from 94.7 to 102.5%, with a variation coefficient of 2.6-8.0%, and the limits of detection and quantification were 1.5 and 5 microg/kg, respectively.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
