Abstract
In the preparation of a group-specific diagnostic antigen for adenoviruses, various procedures for the concentration and purification of the antigen present in the tissue culture fluid of specifically infected cells were investigated. It was found possible to prepare an antigen of increased potency and specificity by passage of the fluid through an ultrafine sintered glass filter, followed by coprecipitation of the antigen with copper hydroxide. This antigen was rendered noninfective by treatment with 0.01% formaldehyde at pH 7 at 37 °C for 4 days or by heating at 56 °C at pH 5.5 for 3 hours. After destruction of the infectivity the antigen was stabilized for storage by lyophilization. This antigen, after such treatment, still retained its serological reactivity.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
