Abstract

A simple simulation sheet drawn a chromatographic column divided into five plates and fifty number cards indicating the amount of a solute possible to exist in the mobile or stationary phase of each plate were prepared. Two rectangular blanks each for indicating the mobile and stationary phases were also drawn on each plate. A simulation for plate theory was performed in the following three steps: (1) only the cards placed on the blanks of mobile phase are moved to that of the mobile phase in the next plate, (2) the two cards, indicating the numbers divided by the sum of numbers on the card(s) placed on each plate according to the distribution coefficient, are selected and replaced on the two blanks of each plate, (3) these steps continue until the fifth plate. This simulation helped students to progress in their learning the mass transfer and mass balance on plate theory in chromatography.

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