Abstract
An antigen-binding fragment (Fab) was prepared against artemisinin (AM) and artesunate (AS) and was developed for use in an enzyme-linked immunosorbent assay (ELISA). The FAb, which was derived from a monoclonal antibody against AM and AS (MAb 1C1) prepared by us, was expressed by Escherichia coli cells, and its reactivity and specificity were characterized. The specificity of the Fab was similar to that of MAb 1C1 in that it showed specific reactivity toward AM and AS only. The sensitivity of the icELISA (0.16µg/mL –40µg/mL for AM, 8.0ng/mL –60ng/mL for AS) was sufficient for analysis of anti-malarial drugs, and its utility for quality control of analysis of Artemisia spp. was validated. The Fab expression and refolding systems provided a good yield of high-quality antibodies. The recombinant antibody against AM and AS provides an essential component of an economically attractive immunoassay and will be useful in other immunochemical applications for the analysis and purification of anti-malarial drugs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
