Abstract
The cyanine dye (1) with three arms of one chromenyl group and two indol groups was synthesized in this paper; and the proposed synthetic mechanism was also mentioned. It has excellent photostability compared to three typical cyanine dyes. Dye 1 gives high chemical activity and nearly turn-on fluorescent response towards hydrosulfide at 600–800 nm, it also has good selectivity towards hydrosulfide over other species; the detection reaction mechanism was proved by 1H NMR analysis, and the emission responses was elucidated by time dependent density functional theory (TDDFT) calculation. The in cellular fluorescent imaging application of the dye 1 was successfully performed for the hydrosulfide imaging of HeLa cells.
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