Preparation of a Novel Microcantilever Array Biochemical Sensor

Abstract

To eliminate the impact of environmental noise such as temperature drift and change of liquid refractive index in the single microcantilever biochemical sensing system and achieve rapid parallel detection of various of target molecules, a novel microcantilever array biochemical sensor was designed and constructed. When the microcantilever array is scanned by a piezoelectric-driven laser beam, the kinetic curves of the specific biochemical reaction on the microcantilever array surface is obtained by real-time monitoring the deflections of the microcantilevers with the optical lever read-out technique. In the experiment, the system optical stability was verified by 9 h scan of two fixed points with 250 mu m pitch, and also the system detection reliability was verified by the temperature excitation test, in which the microcantilever array deflections were basically consistent with each other (error 6.5%) when heating up 6 degrees C. Finally, we detected 10 mu g L-1 of clenbuterol antigen by immobilizing clenbuterol antibody on the gold-coated side of the microcantilever array with the self-made capillary array modification device, which verified the practical feasibility of the microcantilever array sensor.