Preparation of a nano emodin transfersome and study on its anti-obesity mechanism in adipose tissue of diet-induced obese rats

Kun Lu,Qingqing Huang,Shilong Han,Shuanshuan Xie,Shen Qu,Min Tan,Xinwen Chang,Changhui Wang,Lei Xu,Changxing Shen,Haiyan Lin,Jidong Zhang,Xiaolian Song,Jin Chao,Qing Yuan

doi:10.1186/1479-5876-12-72

Abstract

ObjectiveTo describe the preparation of nano emodin transfersome (NET) and investigate its effect on mRNA expression of adipose triglyceride lipase (ATGL) and G0/G1 switch gene 2 (G0S2) in adipose tissue of diet-induced obese rats.MethodsNET was prepared by film-ultrasonic dispersion method. The effects of emodin components at different ratios on encapsulation efficiency were investigated.The NET envelopment rate was determined by ultraviolet spectrophotometry. The particle size and Zeta potential of NET were evaluated by Zetasizer analyzer. Sixty male SD rats were assigned to groups randomly. After 8-week treatment, body weight, wet weight of visceral fat and the percentage of body fat (PBF) were measured. Fasting blood glucose and serum lipid levels were determined. The adipose tissue section was HE stained, and the cellular diameter and quantity of adipocytes were evaluated by light microscopy. The mRNA expression of ATGL and G0S2 from the peri-renal fat tissue was assayed by RT-PCR.ResultsThe appropriate formulation was deoxycholic acid sodium salt vs. phospholipids 1:8, cholesterol vs. phospholipids 1:3, vitamin Evs. phospholipids 1:20, and emodin vs. phospholipid 1:6. Zeta potential was −15.11 mV, and the particle size was 292.2 nm. The mean encapsulation efficiency was (69.35 ± 0.25)%. Compared with the obese model group, body weight, wet weight of visceral fat, PBF and mRNA expression of G0S2 from peri-renal fat tissue were decreased significantly after NET treatment (all P < 0.05), while high-density lipoprotein cholesterol (HDL-C), the diameter of adipocytes and mRNA expression of ATGL from peri-renal fat tissue were increased significantly (all P < 0.05).ConclusionThe preparation method is simple and reasonable. NET with negative electricity was small and uniform in particle size, with high encapsulation efficiency and stability. NET could reduce body weight and adipocyte size, and this effect was associated with the up-regulation of ATGL, down-regulation of G0S2 expression in the adipose tissue, and improved insulin sensitivity.

Highlights

Obesity is recognized as one of the most important public health problems facing the world today
The content of emodin was used as quality control standards and the encapsulation efficiency as evaluation index
Our and other studies have demonstrated that Matrine could reduce the body weight and Lee’s indexes of obese rats, as well as fasting insulin, TG and cholesterol levels [17]

Summary

Introduction

Obesity is recognized as one of the most important public health problems facing the world today. Epidemiological surveys from many countries show that the mean weight of the population is increasing and that the prevalences of clinically-significant overweight and obesity are rising rapidly in adults and, of particular concern, in children and adolescents [1]. Up to one-third of the adults in some westernized countries are obese and over two-thirds in certain smaller populations such as Pacific Islanders; very few countries remain unaffected by obesity [2]. WHO has estimated that 200 million people worldwide were obese in 1995, rising to 300 million in 2000. Revised estimates suggest that 400 million people aged 15 years were obese in 2005, with almost 800 million overweight. By 2015, WHO predicts that these numbers will increase to 2.3 billion overweight and 700 million obese [3]

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