Preparation of a monoclonal antibody and development of an indirect competitive ELISA for the detection of chlorpromazine residue in chicken and swine liver

Abstract

Chlorpromazine is a typical antipsychotic drug used to make food-producing animals calm and promote growth as feed additives. Accumulation of chlorpromazine in animal bodies would cause side effects in the circulatory and nervous systems, and have adverse effects on blood cells, the skin and the eye. To detect the chlorpromazine residue in food producing animals, an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed based on preparation of an anti-chlorpromazine monoclonal antibody. The antibody generated from immunogen of cationic bovine serum albumin (cBSA) coupled with chlorpromazine showed high sensitivity toward chlorpromazine with an IC(50) value of 0.73 ppb. The ELISA method was applied to detect swine liver and chicken samples spiked by chlorpromazine and satisfactory results were obtained. The recovery rates in chicken and swine liver were in the range of 88-95% and 86-95%, respectively; the intra-assay coefficients of variation were both < 15.3% and < 13.5%, respectively. An indirect competitive ELISA method based on a monoclonal antibody towards chlorpromazine with excellent sensitivity and specificity has been successfully developed. The immunoassay provided in this study was a hopeful alternative to chromatography spectrometry for regulatory analysis of chlorpromazine residue in food-producing animals.