Abstract
A new mixed-mode hydrophilic interaction/cation exchange monolithic silica stationary phase was prepared by chemical modification of a monolithic silica skeleton by dextran sulfate (DS) using 3-aminopropyl-triethoxysilane as a cross-linking agent. The characteristics of the DS column were evaluated by the separation of various biomolecules including amino acids, nucleosides and bases, nucleotides, and peptides. Good separations for these polar biomolecules were obtained within 11 min on the column using isocratic mode. The investigation for the separation mechanism indicated that strong hydrophilic and electrostatic interactions combined to play a role in the separation of these biomolecules on the DS column.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
