Abstract
Cottonseed meal protein (CSP) film containing nanoclay and carvacrol was prepared, and the physical properties of the film were investigated. Nanoclay (Cloisite Na+) incorporation improved mechanical properties of the CSP film. CSP film containing 3% nanoclay had tensile strength of 4.07 MPa and elongation at break of 26.37%. The optimal condition for the CSP film manufacturing was 3 g cottonseed protein, 3 g fructose, and 0.09 g Cloisite Na+ in 100 mL of film-forming solution. To inhibit the growth of pathogenic bacteria such as Escherichia coli O157:H7, CSP film containing carvacrol was prepared, and microbial growth was examined during bacon packaging and storage using the film. The antimicrobial activity of the CSP film against E. coli O157:H7 increased with increasing carvacrol concentration. After 10 d of storage, the population of E. coli O157:H7 inoculated on the bacon samples packed with the CSP film containing carvacrol decreased by 1.57 log CFU/g compared to the control. These results suggest that the CSP film containing carvacrol can be used as an antimicrobial packaging film.
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