Abstract
AbstractThe describes an in situ method for the preparation, culture, harvest, and analysis of amniotic fluid samples. In this procedure, cells are grown and harvested for metaphase spreads on glass coverslips. The metaphase spreads are analyzed without being removed from the coverslips. describes a harvesting technique that yields higher‐resolution banding. describes a method for growing more cells by passaging cells from a backup monolayer culture. An describes a flask method for the preparation, culture, harvest, and analysis of amniotic fluid samples that can be used instead of the in situ method. Accompanying is that describes subculturing cells from flasks.
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