Abstract
Animal bone-derived calcium hydroxyapatite (HA) particles were produced and characterized. Adult bovine femoral bone was boiled, washed, cleaned and heated in air at 700°C for 2h. The resulting macro-porous solid was ground, crushed and sieved into particles <50μm.SEM showed the particles were agglomerations of crystals ∼50–500nm across. XRD showed highly crystalline HA with nominal MgO and no detectable CaO. FTIR spectroscopy yielded typical HA absorptions, plus absorptions at 1457 and 1412cm−1 (MgCO3 or CaCO3) and 874cm−1 (CaHPO4). Main elements by EDXRF were Ca and P (molar ratio 1.93 vs. theoretical ratio 1.67). Minor amounts of Si, Mg and Na were detected, plus traces of K, Sr, Zn, Ba, V, Al, Mn, Pb, Cu and Fe. EDX detected Ca, P, Na and Mg. BET gas adsorption surface area was ∼2.23m2g−1 and theoretical particle size ∼857nm. Laser DLS indicated ∼40% of particles were ∼952nm in diameter, plus ∼50% were ∼760nm – in close agreement with BET calculations.By laser Doppler electrophoresis (LDE) the zeta potential of the bone-derived HA particles suspended in 0.154M NaCl was negative for pH 6–11 and −9.25±0.9mV at pH 7.4.Negative zeta potential is reported to favor attachment and proliferation of bone cells. HA particles produced synthetically are reported to have positive zeta potentials. The source of the negative potential was not determined but may stem from factors peculiar to producing HA particles from bone. The results suggest further investigation for biomedical use.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.