Preparation, characterization and wound-healing effect of PEGylated nanoliposomes loaded with oleuropein.

Abstract

Chronic wounds have become a major concern for healthcare systems, as they have been related to diabetic foot ulcers, venous leg ulcers and pressure ulcers. Oleuropein is an active compound that is extracted from olive leaves and it has the ability to reduce injury to tissues owing to its antioxidant effect, hence improving wound healing. The poor pharmacokinetics of oleuropein have limited its use clinically. This work is aimed toward studying the impact of PEGylated and non-PEGylated nanoliposomes loaded with oleuropein, as a carrier model, on wound-healing activity. The thin film hydration method was used to compose PEGylated and non-PEGylated liposomes, both loaded with oleuropein. The results indicated that each free, PEGylated and non-PEGylated composition was within the limit of optimum nanoliposome characterization. The results showed that non-PEGylated compositions produced higher efficiency in encapsulation (47.09 ±10.06%) than the PEGylated ones (20.97 ±10.52%). The PEG-nanoliposomes loaded with oleuropein (PEG-oleu) had mean size, charge and polydispersity index of 129.35 nm, -9.55 mV and 0.1010, respectively. The scratch assay results proved that PEGylated liposomal compositions have a more rapid wound-healing activity than non-PEGylated ones at different time intervals at 0, 2, 24 and 28 h.