Preparation, characterization, and in vitro study of GdSrFe11O19-entrapped poly ε-caprolactone-gelatin scaffolds for inducing the growth of human Wharton's jelly derived- mesenchymal stem cells

Abstract

Nowadays, the development of novel bioactive-containing drug delivery systems (DDSs) plays a vital role in various fields of biomedical science. Therefore, a novel DDS based on gelatin (G)/poly ε-caprolactone (P) nanofibers was developed for efficient delivery of a new hexagonal ferrite (GdSrFe11O19) bioactive to human Wharton's jelly derived-mesenchymal stem cells (hWJ-MSCs). Practically, electrospun PG scaffolds were fabricated using various concentrations of GdSrFe11O19 (2 % w/w (PGH2), 5 % w/w (PGH5), 10 % w/w (PGH10), and 15 % w/w (PGH15)). Next, several analytical devices, including FT-IR, XRD, and FE-SEM, were applied to examine the quality and quantity of fabricated scaffolds. After verifying the quality of manufactured samples by FT-IR and XRD techniques, the size of 200 nm to 230 nm in diameters was ascertained for nanofibers using FE-SEM. The contact angle assay exhibited higher hydrophilicity with optimal nanocomposite concentration for PGH10 than other synthesized scaffolds. The cell viability assay demonstrated that PGH2, PGH5, and PGH10 scaffolds have shown a gradually increased in cell growth values after 3, 5, and 7 days on hWJ-MSCs than the control group, respectively. The cell growth of PGH10 compared to various concentrations of GdSrFe11O19 (including 7.5 µg/ml, 75 µg/m, 150 µg/mL, 300 µg/mL (∼PGH10), and 450 µg/mL) and PGH2, PGH5, PGH10, and PGH15 scaffolds was higher during the treatment period. These data have shown that the PGH10 scaffold would be an efficient platform for the delivery of GdSrFe11O19 bioactive to hWJ-MSCs.