Preparation, Characterization and Function Evaluation of Ultrafine Resveratrol Powder

Abstract

By supercritical SAS,we made the resveratrol anti solvent recrystallization to prepare ultrafine resveratrol powder under supercritical condition. The conditions were under 50℃,20 MPa,resveratrol ethanol solution concentration of 9 mg · m L- 1,and sample solution flow rate of 1 m L · min- 1. We analyzed the particles by Dynamic Light Scattering( DLS) equipment and Scanning Electron Microscopy( SEM). The particle size of the particles was greatly reduced and the average particle size was 0. 68 ± 0. 03 μm. In addition,characterization of ultrafine Res and raw-resveratrol were tested by Fourier transform infrared spectroscopy( FTIR),X-ray diffraction( XRD),High performance liquid chromatography( HPLC) and Liquid chromatographymass spectrometry( LC-MS). SAS process didn't change the chemical structure of Res. The chemical structure of resveratrol was unaffected by the SAS process. The degree of crystallinity of the u-Res particles greatly reduced. We used the purity of the u-Res particles increased from 98. 5% to 99. 2% during the SAS process.Using drosophila anti-aging experiments to detect the oxidation resistance of resveratrol. The antioxidant capacity of resveratrol powder were higher than that in the second-stage and blank experiment under the concentration of resveratrol solution,2. 5,5 and 10 mg · m L- 1. The resveratrol had the oxidation resistance,and ultrafine resveratrol powder was better than raw-resveratrol powder.