Abstract
This chapter provides an overview of the preparation and utilization of an ionspecific calcium mini-electrode. Identification, purification, and subsequent reconstitution into artificial phospholipid vesicles allow the molecular characterization of the mechanisms governing Ca 2+ transport. The use of Ca 2+ -selective electrodes avoids the introduction of dyes into the experimental system as in the case of the spectrophotometric determination of Ca 2+ . The chapter describes the preparation of Ca 2+ -selective mini-electrodes. Ca 2+ -selective membranes are commercially available. Electrode assembly procedure is explained. The chapter discusses how the mini-electrode is assembled by glueing the ionsensitive membrane to the end of a poly vinyl chloride (PVC) tubing. Thermostatted incubation vessel is also illustrated. Optimal application of Ca 2+ -selective mini-electrodes in biochemical experimentation with a need for high sensitivity requires a tailored vessel that should meet some conditions, such as short mixing time. The chapter also examines the use of mini-electrode.
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