Preparation and Release Study of Biodegradable L-lactide IPN’s Insulin Delivery

Athir M Haddad,Hadi S Al Lami,Zainab J Sweah

doi:10.4172/2471-9935.100019

Abstract

Novel triblock copolymers of poly(L-lactide)-poly(ethylene glycol)-sebacate-poly(ethylene glycol)-poly(L-lactide) were synthesized by Ring-Opening Polymerization (ROP) of different ratios of L-lactide with three pre-prepared poly(ethylene glycol)-sebacate-poly(ethylene glycol) polymers A, B and C have different poly(ethylene glycol) molecular weight (2000, 4000, and 10000 g/mole). The triblock copolymers were used to produce nine triblock copolymers having new different lactide chain length (n=10, 25 and 50), coded AL10, AL25, AL50, BL10, BL25, BL50, CL10, CL25, and CL50, respectively. Nine networks were prepared from triblock copolymers with cross-linked sodium alginate, S1-S9. All those polymer networks were loaded with 50 IU of insulin. Each component of the polymer network groups was mixed with cross-linked hydroxyl ethyl cellulose to form SH1-SH9 from S1-S9 fiber IPN’s. These samples were coated with gelatin cross-linked with two different ratios of gluteraldehyde, 20% and 40% w/w by one layer and two layers. Insulin release study at pH 1.2 and 8.4 for 24 hours was performed. Hydrolytic degradation studies were followed at three different pH, 1.2, 7 and 8.4 for 7 weeks with 40% as maximum degradation.

Highlights

There has been an on-going effort to improve the quality of life and find solutions to overcome health problems caused by disease, injury or old age
The continuous request for design and improvement of medical devices, implants, drug delivery systems and artificial organs [1] led to the development and application of a large variety of so-called ‘biomaterials’
Chemicals used in this study were supplied from different sources, poly(ethylene glycol) with average M.wt. 2000, 4000 and 10000, were supplied from BDH Co., sebacoyl chloride and glutaraldehyde were supplied from Fluka Co., 1,2-dichloro ethane (EDC) was supplied from Lab Co., triethylamine was supplied from RDH Co., L-lactide was supplied from BDH Co., Diazabicyclo [5.4.0] undec-7-ene(DBU), diethyl ether and hydroxy ethyl cellulose were supplied from Sigma-Aldrich Co., sodium alginate was supplied from HIMEDIA Co., all materials were used without any purification

Summary

Introduction

There has been an on-going effort to improve the quality of life and find solutions to overcome health problems caused by disease, injury or old age. The continuous request for design and improvement of medical devices, implants, drug delivery systems and artificial organs [1] led to the development and application of a large variety of so-called ‘biomaterials’. The field of controlled delivery of proteins has grown immensely in the last few years. Because of their relatively large size, they have low transdermal bioavailability. Et al [4], produced insulin-loaded PEG/PLA nanoparticles which was biodegradable polymers to be used to improve the surface properties of the system, or coating modification with polymers such as the attachment of poly(ethylene glycol) (PEG) chains to biodegradable polymer such as poly(lactic acid) (PLA), and poly(lactic-co-glycolic acid), PLGA. The hydrophilic PEG chains allow the control of protein and peptide absorption and, will allow regulation of cell behavior at the polymer surface. The U.S Food and Drug Administration have accepted them for internal use in the human body [5]

Methods

Results

Conclusion