Preparation and Recognized Characteristic of Histamine Molecularly Imprinted Polymers

Abstract

HA MIP was prepared in acetonitrile-ethylene glycol mixed solvent ( 20:1，v/v), HA was used as the template, methacrylic acid (MAA) as the functional monomer, azobisisobutyronitrile (AIBN) as the initiator and ethylene glycol dimethaerylate (EGDMA) as the cross-linker. The UV spectrophotometry was used to demonstrate the interaction between HA and MAA. The adsorption characteristics of MIP to HA have been studied by equilibrium binding experiment and Scatchard analysis. The data obtained show that MIP reached equilibrium within 6 h. It is found that within the studied concentration range one HA molecule is entrapped by two MAA molecules The Scatchard chart shows the apparent maximum binding capacity (Bmax) and the dissociation contents (KD) of MIP are 170.5 μmol/g and 0.18 mmol/L, respctively. The MIP synthesized by this method have better binding ability to histamine and can be applied on the separation and detection of histamine.