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Abstract
The recombinant Outer surface protein A (rOspA) from Borrelia burgdorferi is a possible immunogen for protection of infected humans and animals against development of Lyme borreliosis (Lyme disease), a chronic tick-borne disease characterised by diverse dermatologic, neurologic, rheumatic, and cardiac manifestations. For several years, research and development have been directed towards a vaccine for the prevention of this debilitating disease. Numerous animal studies demonstrate that pre-existing antibodies against the outer surface proteins of B. burgdorferi can prevent infection and disease caused by this organism. In this communication, using recombinant DNA technology, genes from B. burgdorferi sensu stricto and B. afzelii were inserted into E. coli-expression vectors and the rOspA were produced. Our aim was to obtain rOspA protein in a purity and quantity desirable for immunization of experimental animals. rOspA is currently the most developed, molecularly-defined vaccine candidate for the prevention of Lyme borreliosis.
Highlights
Lyme borreliosis is a multisystem inflammatory disease caused by Borrelia burgdorferi a gram-negative spirochete[1], transmitted by the bite of infected ixodes ticks
Three species are recognized as pathogenic for humans: all of which are present in Europe: B. burgdorferi sensu stricto, the only species causing Lyme borreliosis in the United States; B. afzelii; and B. garinii
Outer surface protein A (OspA) is abundantly expressed in unfed ticks, probably mediating adherence to midgut cells[5] and enabling borreliae to survive in the vector for prolonged periods without tick feeding
Summary
Lyme borreliosis is a multisystem inflammatory disease caused by Borrelia burgdorferi a gram-negative spirochete[1], transmitted by the bite of infected ixodes ticks. Three species are recognized as pathogenic for humans: all of which are present in Europe: B. burgdorferi sensu stricto, the only species causing Lyme borreliosis in the United States; B. afzelii; and B. garinii. These spirochetes alternate in nature between warm-blooded hosts (mammals) and poikilothermic vectors. OspA is abundantly expressed in unfed ticks, probably mediating adherence to midgut cells[5] and enabling borreliae to survive in the vector for prolonged periods without tick feeding. The switch is in part due to the change in temperature[7]
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Schedule a callMore From: Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
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