Abstract
AbstractPropylproline and N‐demethyl‐lincomycin, two intermediates in lincomycin biosynthesis, were prepared in radiolabeled form. [14C]‐labeled N‐demethyl‐lincomycin was synthesized by a resting cell suspension of a lincomycin negative mutant of Streptomyces lincolnensis that was fed methylthiolincosaminide and uniformly labeled [14C]‐tyrosine. [14C]‐N‐demethyl‐lincomycin was isolated from the culture medium by ion exchange chromatography and reverse phase‐ion pair high pressure liquid chromatography. [14C]‐propylproline was generated by the base hydrolysis of [14C]‐N‐demethyl‐lincomycin and isolated by anion exchange chromatography. The products had radiochemical purities greater than 95% and specific activities of 5.9 mCi/mmole.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Labelled Compounds and Radiopharmaceuticals
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
