Preparation and properties of plasma-initiated graft copolymerized membranes for blood plasma separation

Abstract

A hydrophilic composite membrane for blood plasma separation has been prepared by surface graft copolymerization initiated by low-temperature plasma (LTP). After short LTP pre-irradiation onto a microporous polypropylene (PP) membrane, N-N-dimethylacrylamide (DMAA) vapor was introduced for grafting. The PP membrane had a 0.45 μm effective pore size and a 130 μm thickness. The rate of DMAA grafting onto PP was very high, even in vapor-solid phase reaction under reduced pressure; DMAA 1 mm Hg (133Pa). The percentage of grafted poly-DMAA (PDMAA) reached 15% within 5 min post graft polymerization, and the membrane surface, including the interior surface of pores, became completely hydrophilic. There was no apparent change observed in the membrane morphology in the dry state after the PDMAA-grafted layer was formed. However, water flux significantly decreased, probably due to swelling of the PDMAA-grafted layer. With a grafting yield below 17%, the PDMAA-grafted PP (PP-g-PDMAA) membrane showed a good separation capability of plasma from whole blood. The PP-g-PDMAA membrane exhibited low complement activating potential, high sieving coefficient for plasma proteins and high blood compatibility. Decreases in adsorption of blood cells, plasma proteins, and other biomolecules may be the reason for the membrane performance.