Abstract
The present study reports a specific method for preparation of silver-modified anodic alumina substrates intended for biomaterial applications. Al2O3 coatings were obtained by anodization of technically pure aluminum alloy in sulfuric acid electrolyte. Silver deposition into the pores of the anodic structures was carried out employing in situ thermal reduction for different time periods. The obtained coatings were characterized using scanning electron microscopy (SEM), potentiodynamic scanning after 168 h in 3.5% NaCl solution and bioassays with human fibroblast and NIH/3T3 cell lines. The modified alumina substrates demonstrated better biocompatibility compared to the control anodic Al2O3 pads indicated by increased percent cell survival following in vitro culture with human and mouse fibroblasts. The Ag-deposition time did not affect considerably the biocompatibility of the investigated anodic layers. SEM analyses indicated that mouse NIH/3T3 cells and human fibroblasts adhere to the silver-coated alumina substrates retaining normal morphology and ability to form cell monolayer. Therefore, the present studies demonstrate that silver coating of anodic alumina substrates improves their biocompatibility and their eventual biomedical application.
Highlights
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It is considered that cell growth, cytotoxicity, genotoxicity and biocompatibility depend on the morphological characteristics such as size, shape, surface charge, concentration, distribution of particles, exposure time and cell type [27,28]
In order to determine the concentration of deposited Ag particles into the anodized alumina layers, all samples were submitted to induction coupled plasma optical emission spectroscopy (ICP-OES)
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Various combinations of materials are under in-depth investigations when creating medical devices. It is well known that the quintessential biomaterial has not yet been introduced into the medical practice. In this regard, surface modification of implants is considered to be the most promising method for preventing corrosion and promoting biocompatibility [1]
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