Abstract
A cell-free translation system from Vicia sativa with high activity for reading endogenous messengers was prepared. The concentrations of several of the components were optimized. The system was highly sensitive to cycloheximide, puromycin and fusidic acid, but was completely insensitive to chloramphenicol. The system lacks apparent free endogenous ribosome-inactivating protein activity. It was highly sensitive to several well-known ribosome-inactivating proteins, which makes it very suitable for studying the effects and the mechanism of action of these plant toxins.
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