Abstract
A selenium-modified hyaluronic acid (HA) compound (SeHA) was synthesized as a mimic of glutathione peroxidase (GPX). IR and NMR spectra of SeHA predict that the —CH 2 OH group of the N -acetyl- D -glucosamine in HA was modified by group —SeH. There are averagely 152 —SeH groups in one SeHA molecule. The GPX activities are 103. 88, 32. 15 and 152. 71 U/μmol, respectively, when the substrates were H 2 O 2 , BuOOH and CuOOH. Ping-pong mechanism was observed in the steady-state kinetic studies of the reactive oxygen species (ROS) consuming reaction. Result of reactions of SeHA with free radical capturer 2,6-di- tert -butyl-4-methylphenol(BHT) shows that the catalysis is based on a non-free radical-mechanism and the SeHA can not be inhibited by iodoacetate, an enzyme inhibitor.
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