PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS

Abstract

Objective: In this study, it was aimed to prepare Vancomycin loaded poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles that can be used parenterally in enterococcal infections. Material and Method: In the study, different concentrations of PLGA were used to prepare nanoparticles. Emulsification-solvent evaporation method was used in the preparation of the particles. Particle size and distribution, zeta potential, encapsulation efficiency and yield of the prepared formulations were determined. FTIR analyses were also performed for the formulations. The antibacterial activity was determined as a minimum inhibition concentration by broth microdilution method and as an inhibition zone by disk diffusion method against Enterococcus faecalis ATCC 29212 according to European Committee on Antimicrobial Susceptibility Testing and its effectiveness on bacteria was interpreted by comparing with the pure active substance. Result and Discussion: Particles were successfully prepared by emulsion-solvent evaporation method. Around 300 nm; Particles with a high amount of encapsulated Vancomycin were obtained at PDI values of 0.26-0.28. As a result of the FTIR analysis, it was determined that Vancomycin was loaded into the particle. The MIC values of the prepared PLGA nanoparticles against Enterococcus faecalis ATCC 29212 strain was found to be 4 µg/ml, and the inhibition zone diameters were measured as 15 mm, 15 mm and 16 mm, respectively.