Abstract
Background and aims: The presence of an appropriate scaffold at the wound site could significantly improve the healing process. In this study, we aimed to prepare a biomimetic nanocomposite scaffold composed of chitosan, gelatin, and 58S bioglass nanoparticles for skin tissue engineering. Methods: The nanocomposite scaffolds composed of chitosan, gelatin, and 58S bioglass nanoparticles were fabricated through electrospinning process. Then the cell viability assay was performed in order to evaluate the biological properties of the membranes. The optimum concentration of bioglass nanoparticles was determined for further studies. In vitro characterization was also performed to evaluate physicochemical properties of the scaffolds. Results: The chitosan/gelatin scaffold containing 2% of 58S bioglass nanoparticles showed no cell toxicity, and the dermal fibroblasts were found capable of proliferation on the membrane. The in vitro results obtained from the scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and porosity tests demonstrated the appropriate properties of the membrane as a scaffold for skin regeneration. Conclusions: It was concluded that a chitosan-gelatin membrane containing 2% of 58S bioglass nanoparticles had the potential to function as a scaffold to accelerate wound healing due to its suitable properties, such as high porosity, high surface/volume ratio, and excellent biocompatibility.
Highlights
Skin is the largest organ of the body and the first line of defense against external pathogens
scanning electron microscopy (SEM) and dynamic light scattering (DLS) analyses of the synthesized 58SBG-NPs The results obtained from SEM and DLS revealed the morphology, average size, and size distribution of the 58SBG-NPs
The ATRFTIR spectra showed the characteristic bands of CS, GEL, and Bioactive glasses (BGs)-NPs, proving the successful incorporation of 58SBG-NPs into the nanofibrous scaffold
Summary
Skin is the largest organ of the body and the first line of defense against external pathogens. Autologous grafting as the main treatment provides a good chance for healing, it cannot be always used due to its many limitations such as patients suffering, scar formation, slow rate of healing, and limited donor sites [2] In this regard, allografts and xenografts are considered as alternative methods of treatment. We aimed to prepare a biomimetic nanocomposite scaffold composed of chitosan, gelatin, and 58S bioglass nanoparticles for skin tissue engineering. Conclusions: It was concluded that a chitosan-gelatin membrane containing 2% of 58S bioglass nanoparticles had the potential to function as a scaffold to accelerate wound healing due to its suitable properties, such as high porosity, high surface/volume ratio, and excellent biocompatibility.
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