Abstract

Preparation of theShigella flexneri 2a lipopolysaccharide labelled with32P is described. For the extraction we used a mixture of phenol and water (1∶1) at 65–69°C. Ballast substances were removed by precipitation with trichloracetic acid and by ethanol. The preparation was checked by agar electrophoresis. Ouchterlony's diffusion and immunoelectrophoresis. Its behaviour during gel filtration on a column of immunosorbent prepared by binding immune gamma globulin to Sephadex G-25 modified chemically by introducing a CN(-) group through the action of cyanogen bromide, was studied.

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