Abstract
The retention and selectivity of bufadienolides (cinobugain, resibufogenin, bufalin) in Bufonis venenum were studied using high-performance liquid chromatography on octadecyl- or phenylpropyl-treated porous glass. From elemental analysis data for carbon, the maximum number of bonded octadecyl or phenylpropyl surface groups per 100 Å 2 of glass (mean pore diameter 157 Å, specific surface area 213 m 2/g) in octadecyl or phenylpropyl gel was calculated to be 2.15 and 1.97, respectively. Using acetonitrile—water mixtures as the eluent, bufadienolides were separated on octadecyl or phenylpropyl gels, but with different degrees of resolution.
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