Abstract
Several kinds of fatty acid esters of fluorescent p-substituted phenols (1a, 2a, 3a, and 4a) were prepared. Their absorption and fluorescence spectral properties and their usefulness as substrates for the measurement of lipase activity were investigated. Among them, long alkyl chain esters were found to be suitable for fluorescent substrates, because their emission wavelengths were fairly different from those of the corresponding phenols. In particular, 4- (2-benzothiazolyl) phenyl myristate (3e), 4- (2-benzothiazolylvinyl) phenyl laurate (4d) and 4- (2-benzothiazolylvinyl) phenyl myristate (4e) were ascertained to be easily hydrolyzed by porcine pancreatic lipase.
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