Preparation and evaluation of chitosan skin patches containing mesoporous silica nanoparticles loaded by doxycycline on skin wound healing.

Abstract

This study aims to prepare and evaluate a skin patch containing mesoporous silica nanoparticles with doxycycline for skin wound healing in a rat model. For this purpose, 84 female rats were randomly placed in four equal groups: (A) Control group with only skin defects and no therapeutic intervention; (B) Chitosan group in which a chitosan skin patch without loading any drug was placed on their skin defect; (C); The ChMesN group had a chitosan skin patch containing drug-free mesoporous silica nanoparticles; (D) ChMesND group had a skin patch loaded with doxycycline loaded with mesoporous silica nanoparticles on their skin defect. The histological results showed that on the 3rd day of the study, collagen fiber orientation was significantly higher in the ChMesND group than in the other groups. On the 7th day of the study, neovascularization, and inflammation in the ChMesND group were significantly higher and lower than in the other groups, respectively. On day 21, the most re-epithelialization was observed in the ChMesND group. It was found that on day 7, the wound area in the ChMesND group was significantly less than in other groups. On the 21st day of the study, the minimal experimental wound area was related to chitosan and ChMesND groups. Although chitosan has anti-inflammatory effects, its combination with doxycycline with several beneficial biological effects can have significant therapeutic effects with chitosan. Hence, it can be concluded that chitosan skin patch containing doxycycline can be suitable dressings for managing and accelerating the healing of skin wounds.