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Abstract
The walnut shell biochar was prepared at high temperature in this experiment. The effect of different biochar on the growth of Bacillus thuringiensis (Bt) were investigated, and biochar with good biocompatibility to Bt was selected and used as an adsorbent to prepare Bt/ sodium alginate/ biochar composite microspheres. The effects of sodium alginate, calcium chloride mass fraction, and biochar addition on the encapsulation rate and the spheroidization rate of microspheres were investigated. The results showed that the optimum preparation conditions were as follows: the mass fractions (w/v) of sodium alginate, biochar, and calcium chloride were 3%, 0.4%, and 2%, respectively. The encapsulation rate and sphericity rate of prepared composite microspheres were 93.50% and 90%, and the diameter was about 1200 μm after drying. Release behavior in water showed that Bt-sodium alginate-biochar microspheres had better sustained release performance than those without biochar.
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