Preparation and Evaluation of Antioxidant Activities of Bioactive Peptides Obtained from Cornus officinalis.

Xin Zhang,Pengfei Jiao,Bo Tang,Mengrong Xia,Hao Zhang

doi:10.3390/molecules27041232

Abstract

The present study is a preparation of bioactive peptides from Cornus officinalis proteins by the compound enzymatic hydrolysis method. Response surface methodology (RSM) coupled with Box–Behnken design (BBD) is used to optimize the preparation process of Cornus officinalis peptides. The effects of independent variables, such as the amount of enzyme, pH value, time, extraction times and the ratio of material to liquid on the yield of peptides, are also investigated. The analysis results of the RSM model show that the optimum conditions for the extraction of Cornus officinalis peptides were a pH value of 6.76, temperature of 48.84 °C and the amount of enzyme of 0.19%. Under optimal conditions, the yield of peptides was 36.18 ± 0.26 %, which was close to the predicted yield by the RSM model. Additionally, the prepared Cornus officinalis peptides showed significant antioxidant activity; the scavenging rates of the peptides for DPPH and ·OH were 48.47% and 29.41%, respectively. The results of the cell proliferation assay revealed that the prepared Cornus officinalis peptides could promote embryo fibroblast cells proliferation and repair oxidative damage cells. These results have a practical application value in the design of novel functional food formulations by using Cornus officinalis.

Highlights

Cornus officinalis (Cornus officinalis Sieb.et Zucc.) is classified as a tonic in traditional Chinese medicine
The results showed that the combination of papain and trypsin enzymes (A:B) had the best hydrolysis effect on Cornus officinalis protein with the degree of hydrolysis (DH) was 32.62%
The actual extraction of the yield of peptides was recorded as 36.18 ± 0.26 %, which was highly consistent with the predicted yield. These results showed that the Response surface methodology (RSM) model has high reliably and accurately predicts the relationship between the preparation variables and the yield of peptides

Summary

Introduction

Cornus officinalis (Cornus officinalis Sieb.et Zucc.) is classified as a tonic in traditional Chinese medicine. There are various biologic active compounds, including iridoids, tannins, flavonoids, polysaccharides, triterpenoids, ursolic acid and organic acid esters, are present in Cornus officinalis [1,2,3]. There are many studies on the extraction of polysaccharides, phenolic acid compounds, diverse iridoid glycosides, saponins, tannins and other physiological active substances from Cornus officinalis [9,10,11,12]. There are only a few studies on how to use the Cornus officinalis protein [13,14,15]. The protein resources in Cornus officinalis are often wasted during the extraction process. It is significant to develop an effective strategy to utilize protein resources from Cornus officinalis

Methods

Results

Conclusion