PREPARATION AND EVALUATION OF ADJUVANTED LIVE VACCINE AGAINST INFECTIOUS BRONCHITIS

Abstract

live vaccines are widely used in poultry industry. Such vaccines can be either injected or delivered onto the bird mucosa and most probably not adjuvanted. The herein study was devoted to focus on the potential role of gel adjuvant when added to infectious bronchitis live attenuated vaccine (IB). Montanide gel 01 is a polymer adjuvant that was recommended to be added to live vaccines improving their immunogenicity and efficacy. It was noted that augmentation of IB live vaccine with Montanide gel 01 resulted in superior quality vaccine when utilized as spray as well as the intranasal methods. Both humoral and cellular immunity were higher upon using the IB- Montanide gel 01 more than the conventional non- adjuvanted IB vaccines. Moreover, the gel 01 IB vaccine showed higher protection than the ordinary vaccines when challenged with the virulent IB strains. It was also demonstrated that gel IB vaccine could be successfully used as spray and intranasal delivered vaccine especially in mass vaccination in huge poultry industry. The results also proved that the use of Montanide gel 01 vaccine increased the safety margin of the IB vaccine and enhanced the IB vaccine efficacy.